Get ready to explore the microscopic world with unprecedented clarity! The xView module is a game-changer for multiphoton microscopy, pushing the boundaries of what we can see and study.
The xView Revolution:
Bruker's xView™ Module is an innovative tool for the Ultima 2Pplus multiphoton microscope, offering a groundbreaking approach to high-resolution imaging. It expands the field of view by a staggering 2.5 times, allowing researchers to capture intricate neural activity and structural intricacies across vast brain regions without sacrificing speed or sensitivity. But here's where it gets exciting: it achieves this without the usual trade-offs!
No Compromises:
Traditionally, increasing the field of view meant accepting lower resolution due to reduced magnification. But xView challenges this limitation. Researchers can now enjoy the best of both worlds: the wide field of view of a 10X objective and the high-resolution power of a 16X lens. This is made possible by Bruker's patented ETL compensation, which corrects aberrations in real-time, ensuring sharp images even at large scales.
Unleashing New Possibilities:
Designed for advanced neuroscience research, xView opens doors to groundbreaking studies. It enables brain-wide connectivity analysis, circuit mapping, and disease research with unparalleled precision. Moreover, when combined with Bruker's NeuraLight 3D Ultra® Module, researchers can perform simultaneous large-area imaging and optogenetic stimulation, offering a dynamic view of neuronal networks.
Technical Excellence:
- Patented ETL Compensation: Seamlessly integrates with the ETL module for aberration correction, ensuring crisp images at large scales.
- Optogenetics Ready: Compatible with NeuraLight 3D Ultra SLM for optogenetic experiments.
- High-Resolution Imaging: Captures stunning details with up to 8K x 8K pixels in galvo mode and 5K x 5K in resonant mode, powered by xCore FPGA electronics.
- Flexible Lens Sets: Researchers can choose from FN 28, 36, or 45 mm equivalent lenses, tailoring the setup for specific experiments.
Expand Your Imaging Horizons:
The user-friendly lens sets allow researchers to strike the perfect balance between resolution and field of view. When operating in large FOV mode, xView utilizes the ETL module to maintain image quality, all managed through Prairie View software. This setup redefines the imaging experience, providing high-speed, high-resolution imaging across extended areas.
Imaging at the Next Level:
As an upgrade for the Ultima 2Pplus, xView enhances imaging capabilities, making it ideal for tracking cellular processes in previously hard-to-reach regions. It boosts throughput for large tissue scans, eliminating the need for image stitching. With Ultima xCore FPGA electronics, xView delivers frame rates up to kilohertz for regional imaging and supports 8K resolution for detailed studies.
Applications Unbound:
The xView module, when paired with the Ultima 2Pplus or integrated with other techniques, enables a range of cutting-edge neuroscience applications:
- 3D Optogenetics and Imaging: Simultaneously study neural inputs and outputs with large-area imaging.
- Enhanced Tissue Montages: Create comprehensive tissue maps with improved efficiency.
- Mesoscale Circuit Mapping: Uncover neural circuits with enhanced temporal and spatial resolution.
- Disease Pathway Analysis: Investigate neural diseases like Alzheimer's, epilepsy, and Parkinson's.
- 3D Cell Culture Imaging: Visualize complex cell cultures and large organoids.
Specifications:
- FOV Modules: FN 28, 36, and 45 mm equivalent lens sets for versatile imaging.
- Wavelength Range: 400 to 1300 nm, with >90% transmission at 700 to 1100 nm.
- Requirements: Ultima 2Pplus microscope, xCore electronics, ETL module, resonant scanner, and compatible objectives.
- Objective Compatibility: Supports Olympus, Nikon, and other infinity-corrected confocal/multiphoton objectives with RMS, M25, M27, and M32 threading.
Example Performance:
- Olympus 20X 1.0 NA objective with xView 36: FOV of 1.27 x 1.27 mm
- Nikon 16x 0.8 NA objective with xView 45: FOV of 2.2 x 2.2 mm
And this is just the beginning! The xView module is a powerful tool that invites researchers to explore new frontiers in neuroscience imaging. Are you ready to unlock the secrets hidden within the microscopic realm? The possibilities are endless, and the scientific community is eager to hear your thoughts on the potential of this groundbreaking technology.
